13040800595

Aquatic antifouling varnish

Aquatic antifouling varnish

Butenolide antifouling agent
Butenolide Antifoulant

butenolide ™ It is a small molecule of biological signal with antifouling effect released by marine microorganisms to prevent the competition of fouling organisms. Use butenolide ™ Technology can help the hull and marine facilities to naturally form a layer of bioactive protective film coating in the sea water, strengthen the antifouling activity of the surface coating of the hull and marine facilities, and effectively enhance the antifouling effect by using butenolide ™ Technology, can reduce the use of paint and reduce maintenance costs for you.

Features and benefits

It is applicable to all types of offshore installations and ships of various speeds;

Due to patented butenolide ™ Bionics technology, especially suitable for slow ships or static marine facilities;

Effective against global fouling organisms, applicable to any sea area;

Match with a variety of resins, micro addition can significantly enhance the antifouling effect;

The most matched effective duration of antifouling can be obtained based on the linear polishing rate, up to 90 months without cleaning;

Reduce robot cleaning and maintenance costs by 75%.

Technical report on natural non-toxic butenolide antifouling agent

1. Laboratory anti fouling biological larvae adhesion activity test

The adults of barnacle Balanusamhite Darwin, polychaete coiled tube worms Hydroideselegans Haswell and bryozoan Bugula neritina Linnaeus were collected from the rafts and rafts in the Niuwei Sea (22o 19'N, 114o 16'W) in Hong Kong. After the collected barnacles are cleaned and dried in the shade, they are put into sterilized seawater for aeration culture. After the pool, barnacles larvae are released. Nauplii larvae are collected from the larvae to the light, and transferred to fresh seawater to add diatom Chaetoceros gramilis and a small amount of antibiotics for feeding. The culture temperature is 28 ° C. The seawater is replaced every day to supplement the bait algae. After 3-4 days of culture, barnacles larvae can develop to the Cyprid stage of Venus larvae, Cyprids were collected for larval attachment activity test.

After the collection, healthy male and female worms are selected. Under the anatomical microscope, gently stimulate the head of the adult worms with tweezers to release sperm and eggs. If necessary, the worms can be gently pulled out of the tube to facilitate the collection of eggs and sperm. After fertilization, the fertilized eggs are collected and cultured in the incubator using the same culture method as the barnacle larvae, and the diatom Isochrysis galbana is fed, After about 5 days, collect a small amount of larvae and place them on the natural biofilm. If the larvae attach one hour later, collect the remaining larvae for the attachment activity test. In the experiment of larval attachment activity, because the coiled tube worm needs an inducer to attach, 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor of 10-4M concentration, should also be added to the sample as an artificial inducer. Observe the attachment and metamorphosis of larvae 24 hours later.

Adults of Carex racemosus were collected and put into seawater to avoid light. The next day, they can be taken out and put into seawater to collect larvae with light, and then put into samples for activity test. The liverworts attach very quickly, and operate as quickly as possible to avoid introducing errors.

In the above larval activity and attachment activity test, the compound was first prepared into 50 μ g/μ L with DMSO, and then the compound was prepared into a series of samples with concentrations of 100, 50, 25, 12.5, 6.2, 3.2, 1.6, 0.8, 0.4 μ g/ml with seawater filtered with 0.22 um filter membrane by double dilution method. Then one milliliter of sample was added into each hole of the sterile 24 hole polystyrene board, and 10-20 larvae were put in, Three parallel controls were established. The attachment state of larvae was observed and analyzed at 28 degrees. After a certain period of time, the attached larvae will be counted and expressed in percentage. At the same time, the death of larvae was observed. The results are shown in Table 1, and the data shows SEASAFE ™- The activity of 021 was significant.

2. Performance evaluation of Butenolide (Butenolide) based antifouling coating

For the complex and diverse marine environment, real sea assessment is the most accurate and authoritative way, and also a very necessary means. We will take SEASAFE ™- 021 was compounded with self polishing antifouling polymer (market leading polymer) to prepare marine antifouling coating. The hanging plate experiment was carried out on the floating raft in Hong Kong Niuwei Sea (22o 19'N, 114o 16'W), and regular sampling observation was conducted to evaluate its antifouling performance. Figure 2 shows self polishing antifouling polymer/SEASAFE ™- According to the marine hanging plate experiment results of 021, the three pieces on the left are the control group, and the surface has been seriously polluted by marine organisms in about six months. And SEASAFE on the right ™- 021 antifouling coating compounded with silicon polyacrylate, zinc polyacrylate and copper polyacrylate self polishing antifouling polymer, with the addition content of only 5 wt%, it can be seen that by selecting appropriate self polishing polymer and SEASAFE ™- 021 composite, can achieve up to 1 year of anti pollution effect. In addition, SEASAFE ™- 021 is compounded with the current mainstream antifouling coating (Figure 3), which can effectively improve Its anti pollution ability.

Fig. 3 Self polishing antifouling polymer/SEASAFE ™- 021 Marine hanging plate experiment of composite antifouling coating (copper free formula on the left, mainstream cuprous oxide formula on the right)

SEASAFE ™- 021 is a potential antifouling compound with outstanding advantages. (1) Simple structure, easy chemical synthesis and mass production; (2) The polarity is very small, easy to mix with paint, and will not be released into sea water soon, and the dilution is controllable.

3. Environmental Risk Assessment of Butenolide

In the Biological Products Regulation (BPR) issued by the European Union, it is stated that a promising antifouling agent should meet the following three requirements at the same time: (1) rapid degradation in different environmental media to avoid accumulation and pollution in the environment caused by large-scale application; (2) Low toxicity to non target organisms, ensuring that the release of antifoulant will not affect the ecological balance; (3) It has good antifouling activity and can effectively inhibit the attachment of fouling organisms.

Because of SEASAFE ™- The outstanding performance of 021 in the field test has shown us the potential market prospect of this compound. In order to better understand whether this compound has real market development value, we compare it with SEA-NINE, an anti fouling compound on the market ™ The activity and toxicity of DCOIT, the main component of 211N, were compared. SEA-NINE ™ 211N is an anti fouling product developed by Rohm and Hass of the United States for many years, which is characterized by low toxicity and fast degradation. However, it has been detected that the anti fouling agent has accumulated in environmental media. Our compounds and SEA-NINE ™ The structure of 211N is very similar, but SEASAFE ™- The structure of 021 is simpler, without chloride and sulfur ions, so we use DCOIT (SEA - NINE) with 98% purity ™ 211N) as a positive control ™- 021, the study found SEASAFE ™- 021 can be rapidly degraded with a half-life of 13 hours. However, DCOIT did not degrade significantly after 96 hours. The current experimental data shows that SEASAFE ™- 021 will be rapidly degraded by organisms in marine water bodies in the environment, and its half-life is faster than SEA-NINE ™ 211N (Fig. 4). SEA-NINE has been detected in the environment so far ™ The maximum concentration of 211N is 3 μ G/L, more excellent biodegradability indicates SEASAFE ™- 021 will not accumulate to high concentration (>3 μ g/L)。 In the subsequent experiments, seawater filtered by different pore size filter membranes (100um, 2um, 1um and 0.22um) was used to carry out degradation experiments. It was found that bacteria in SEASAFE ™- 021 played a major role in the degradation process. In addition, collected in SEASAFE ™- 021 The seawater at different stages of degradation was distracted by the antifouling experiment. The experiment showed that the attachment rate of barnacle larvae increased with the increase of SEASAFE ™- The degradation of 021 gradually increased, and the growth inhibition ability of marine diatoms also increased with SEASAFE ™- 021, which indicates that SEASAFE ™- The degradation of 021 will not produce more toxic by-products, and the non-toxic environment will accumulate risks.

Fig. 4 SEASAFE ™- Comparative analysis of biodegradation and acute toxicity between 021 and DCOIT

To evaluate SEASAFE ™- 021 For the toxicity of non target organisms, we used Oryzias melastigma as the model organism and conducted a 28 day chronic exposure toxicity experiment. Male and female adult fish exposed to low concentration of SEASAFE ™- 021 (0, 0.3, 1 and 3 μ G/L), and DCOIT of environmental concentration is taken as positive control (3 μ g/L)。 A series of sensitive biomarkers, involving liver oxidative pressure, neural signal transduction, endocrine interference and reproductive function, are used to measure SEASAFE ™- Relative biosafety of 021. The results show that compared with DCOIT, SEASAFE ™- 021 The oxidative pressure caused by chronic exposure to male and female liver is relatively low, and can quickly return to normal level (Figure 5). In addition, DCOIT exposure significantly increased the content of estrogen in male serum and decreased the content of testosterone in serum, which proved that DCOIT is a strong endocrine disruptor. Phase to ground, SEASAFE ™- 021 has a much smaller interference effect on the endocrine system. At the same time, the chronic exposure of DCOIT inhibited the reproductive function of medaka, which was manifested in the decline of egg production, the low hatching rate of young offspring and the decline of motor ability. SEASAFE ™- 021 had no effect on the end point indicators, including the number and quality of eggs, indicating that it would not affect the reproductive function of fish. Based on the above results, we can see SEASAFE ™- 021 has relatively weak toxicity to non target organisms and can gradually recover. The true dose of DCOIT in the environment can interfere with the endocrine system and damage the reproductive function of non target organisms. The subsequent results of liver proteomics further verified this point. We found that DCOIT can significantly induce the expression of vitellogenin VTG in the liver of male fish, and increase the content of VTG in the serum of male fish. VTG is a widely used sensitive biomarker, which can effectively evaluate the endocrine disrupting effects of compounds. Therefore, the induction of male VTG by DCOIT shows that it has estrogenic activity. However, SEASAFE ™- The chronic exposure of 021 did not affect the level of VTG in the liver and serum of medaka, indicating that it would not interfere with the balance of endocrine system. The result of environmental risk assessment shows that, compared with DCOIT, our Seasafe product SEASAFE ™- 021 has obvious advantages such as easy degradation and low toxicity.

Fig. 5 SEASAFE ™- Comparative analysis of chronic toxicity between 021 and DCOIT

The above technical introduction materials are compiled based on the scientific research tests and published papers of the research team of Professor Qian Peiyuan's laboratory of the Hong Kong University of Science and Technology in recent years. It is only used as the data for Haike to understand the technology for its partners, not a technical guarantee. With respect to the impact of health, safety, environment and other aspects involved in the use of the product, the product user/employer shall comply with the corresponding regulations during the operation. Haike Biotechnology Co., Ltd. is not responsible for any damage caused by any operation or contact with the above products.